This research, "Ethnobotanical Survey and Pharmacognostic Profiling of Medicinal Plants Used in Wound Healing," sets out to record the traditional medicinal plants utilized to heal wounds and scientifically assess their pharmacognostic attributes. Carried out in a chosen rural area in India, the research utilizes an ethnobotanical survey using formal interviews with traditional practitioners and local healers to determine popular plant species in use. 25 medicinal plants were screened and examined with macroscopic, microscopic, physicochemical, and phytochemical screening for their bioactive potential. Results show that the most common phytochemicals are flavonoids (92%) and tannins (88%), responsible for tissue regeneration and antimicrobial activity. Their anti-inflammatory and antiseptic activities are brought about by alkaloids (76%) and saponins (68%). Statistical analysis also disclosed a high correlation (r = 0.85) between the concentration of flavonoids and antimicrobial activity that testifies to their therapeutic significance. Results vindicate scientifically the age-old practice of using plants as drugs in wound healing and establish a basis for their incorporation into contemporary herbal preparations and drug development.