An uptick in research into green synthesis methods, especially those that make use of plant extracts, can be attributed to the rising need for sustainable and environmentally friendly nanomaterials. Antibacterial activity against Staphylococcus aureus and Escherichia coli was assessed in this study, which also looked at the green manufacture of metallic nanoparticles using Ocimum sanctum herbal extract. Analyses using UV-Visible spectroscopy, FTIR, SEM, and XRD verified the successful production of nanoparticles. S. aureus was more susceptible than E. coli in antibacterial experiments, which showed a noticeable rise in inhibitory zones that was dose dependent. The produced nanoparticles' powerful antibacterial effectiveness was confirmed by statistical analysis employing one-way ANOVA, which showed significant differences (p