Skip to main content
editor@ijphdt.com
9343055451
e-ISSN: 3049-1630
International Journal of Pharmacognosy and Herbal Drug Technology

International Journal of Pharmacognosy and Herbal Drug Technology

Published

Anti-Inflammatory Activity of Herbal Extract Using Albumin Denaturation Method

Published in IJPHDT, Vol-3, Issue-05, June-August: 2026 (Vol. 3, Issue 5, 2026)

Anti-Inflammatory Activity of Herbal Extract Using Albumin Denaturation Method - Issue cover

Abstract

Inflammation is a protective biological response against harmful stimuli such as pathogens, tissue injury, and toxic substances. However, chronic inflammation may lead to several diseases including arthritis, cardiovascular disorders, and cancer. Conventional anti-inflammatory drugs are effective but often produce adverse effects during long-term use. Therefore, herbal medicines have gained importance as safer alternatives due to the presence of bioactive phytochemicals with anti-inflammatory potential. The present study was carried out to evaluate the in vitro anti-inflammatory activity of selected herbal extracts using the albumin denaturation method. Herbal drugs such as turmeric (Curcuma longa), neem (Azadirachta indica), tulsi (Ocimum sanctum), and ginger (Zingiber officinale) were selected for the study. The extracts were prepared using Soxhlet extraction with suitable organic solvents. Different concentrations of the extracts were tested against heat-induced denaturation of albumin protein and compared with the standard drug diclofenac sodium. The anti-inflammatory activity was assessed by measuring the percentage inhibition of protein denaturation at 660 nm using a UV-visible spectrophotometer. Among the tested extracts, neem and turmeric showed significant inhibition of albumin denaturation, indicating appreciable anti-inflammatory activity, although lower than the standard diclofenac sodium. The activity may be attributed to the presence of phytoconstituents such as curcumin, flavonoids, tannins, gingerols, eugenol, and limonoids. The study suggests that these herbal extracts possess promising anti-inflammatory potential and may serve as natural therapeutic agents for inflammatory disorders. The albumin denaturation assay proved to be a simple, economical, and reliable method for preliminary screening of herbal anti-inflammatory activity.

References

  1. [1]-Incubate at 37ºC for 20 minutes . Tulsi extract also demonstrated moderate inhibition of albumin denaturation, with a maximum inhibition of 52.75%. The anti-inflammatory effect of tulsi may be due to phytoconstituents such as eugenol, rosmarinic acid, ursolic acid, and flavonoids, which possess antioxidant and anti-inflammatory activities.
  2. [2]Pipetting should be done carefully to avoid volume errors.
  3. [3]Air bubbles should be avoided while filling cuvettes. Abdelhamid H. Elgazzar and Magda Elmonayeri. (Inflammation) Mizushima, Y., & Kobayashi, M. (1968). Interaction of anti-inflammatory drugs with serum proteins, especially with some biologically active proteins. Journal of Pharmacy and Pharmacology. Medzhitov R. Inflammation 2010: new adventures of an old flame. Cell. 2010;140:771–776. Nathan C, Ding A. Nonresolving inflammation. Cell. 2010;140(6):871–882. Abdulkhaleq LA et al. The crucial roles of inflammatory mediators in inflammation. Vet World. 2018;11(5):627–635. Mizushima, Y., & Kobayashi, M. (1968). Interaction of anti-inflammatory drugs with serum proteins, especially with some biologically active proteins. Journal of Pharmacy and Pharmacology, 20(3), 169–173. Vane, J. R., & Botting, R. M. (1998). Anti-inflammatory drugs and their mechanism of action. Inflammation Research, 47(2), 78–87. Sakat, S., Juvekar, A. R., & Gambhire, M. N. (2010). In vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn. International Journal of Pharmacy and Pharmaceutical Sciences, 2(1), 146–155. Sakat, S., Juvekar, A. R., & Gambhire, M. N. (2010). In vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn. International Journal of Pharmacy and Pharmaceutical Sciences, 2(1), 146–155. Rasu R., Blessina S., & Sangeetha N. (2025). Comparative Profiling of Phytochemical and Nutritional Constituents in Neem, Gooseberry and Tulsi Leaves. International Journal of Current Microbiology and Applied Sciences, 14(11), 12–19. Kela E, Sogbesan AO, Wakil UB. Evaluation of Phytochemical Composition of Ginger Extracts. Fisheries and aquaculture journal. 2021 Dec 30;12(7) Sherly E.A., Prabhat Kumar, Pawan Kumar, Tripathi A., Sengar P., & Tiwari S. (2023). Role of Five Medicinal Plants (Giloy/Guduchi, Garlic, Tulsi, Turmeric and Ginger) in Human Immune System. International Journal of Innovative Science and Research Technology, 8(2). Chandra, S., & Chatterjee, P. (2012). Evaluation of anti-inflammatory effect of ashwagandha: A preliminary study in vitro. Pharmacognosy Journal, 4(29), 47–49. Popuri, A. K., & Pagala, B. (2013). Extraction of curcumin from turmeric roots. International Journal of Innovative Research and Studies, 2(5), 290–299. Rajesh Kumar Nema ,S.N meyanathan Chandra Shekhar , Mahesh Verma , C.S Bhan , “A practical approach to Pharmaceutical Analysis” CBS Publishers and distributer Pvt. Ltd , First edition – 2008 , Reprint -2011 page no. 1-3 .

Authors (4)

Majid Shabbir Khan

Pharmaceutical Chemistry Depar...

View all publications →

Pankaj Rangnath Bharsat

Pharmaceutical Chemistry Depar...

View all publications →

Pari Sanjay Bhavsar

Pharmaceutical Chemistry Depar...

View all publications →

Mohan Balasaheb Bute

Pharmaceutical Chemistry Depar...

View all publications →

Download Article

PDF

Best for printing and citation

File size: 2.1 MB
Format: PDF

Summarise this paper with AI:

Article Information

IJPHDT530008

IJPHDT-01-000246

102-127

2026-08-04

JATS XML

Article Impact

Views:4,369
Downloads:578
scite_
PlumX Metrics Badge

How to Cite

Shabbir, M., & Rangnath, P. & Sanjay, P. & Balasaheb, M. (2026). Anti-Inflammatory Activity of Herbal Extract Using Albumin Denaturation Method. International Journal of Pharmacognosy and Herbal Drug Technology, 3(5), 102-127. DOI:https://doi.org/10.64063/3049-1630.vol3.issue5.000246

Article Actions

Whatsapp