Zebrafish

Medicinal plant research has gained immense importance due to the increasing demand for plant-derived therapeutics. The integration of small organism models and protein quantification techniques has significantly enhanced the understanding of pharmacological activities, toxicity, and molecular mechanisms of bioactive compounds. Small organisms such as zebrafish, Drosophila melanogaster, and Caenorhabditis elegans serve as cost-effective and genetically tractable models for in vivo studies. Concurrently, protein quantification methods like Bradford, Lowry, BCA, ELISA, and advanced proteomic techniques provide insights into molecular interactions and bioactive compound pathways. This review critically compares these models and techniques, highlighting their advantages, limitations, and applications in medicinal plant research.