Preclinical Studies

Gingivitis is a more common and reversible periodontal disease that mostly happens due to dental plaque build-up and such a periodontal disease is mostly treated using chemical mouthwashes like chlorhexidine, but despite its use in the prevention of the disease, the use of such type of mouthwashes is usually linked to several undesirable side effects. It was a multi-center randomized controlled clinical trial that assessed the safety and effectiveness of herbal mouthwash standardized to prevent gingivitis. Two hundred individuals who had mild to moderate gingivitis were selected in three dental care facilities and then randomly allocated into either the herbal mouthwash or control group to undergo 12 weeks of intervention. Clinical results were measured with the help of the Gingivar Index (GI) and the Plaque Index (PI) at baseline, 4, 8, and 12 weeks and the safety and tolerability measured with the structured adverse-event reporting. The findings showed statistically significant and progressive depressing effects of the herbal mouthwash group on both GI and PI scores relative to the control group at all intervals of the follow-up (p

Ayurvedic polyherbal formulations are increasingly explored internationally as a budding field of therapeutic potential based on thousands of years of traditional medicinal practice. However, the absence of standardization protocol and stringent quality control measures is a significant barrier, and the adoption of their safe and effective use in western healthcare is a challenge. The goal of this review is to evaluate the standardization and quality control measures related to Ayurvedic polyherbal formulations. The review discusses the complexity of multi-component herbal mixtures, variability of raw material and absence of marker compounds and synergistic effects which make reproducibility more difficult. The review emphasizes the use of modern techniques including High-Performance Thin Layer Chromatography (HPTLC), Liquid Chromatography–Mass Spectrometry (LC-MS) methods, and microbiological analyses, all increasingly common approaches to assess the variance in products. While there have been advances in the use of HPTLC and LC-MS for standardization and quality control, many regulatory frameworks do not exist, and harmonized quality standards are absent. The review indicates a need for the traditional Ayurvedic approach and modern science to work collaboratively to develop standardized strategies. A successful collaborative effort would lead to increased global/consumer recognition, acceptance and contribution to enhanced public safety regarding the quality and efficacy of these established therapeutic preparations.